The present study demonstrated, by immuno- histochemistry and Western blotting, the presence of im- munoreacting atrial natriuretic peptide (ANP) and endo- thelin in the heart of Protopterus annectens by both light and electron microscopy. The distribution of ANP gran- ules was investigated. ANP granules were localised in myocytes from the atrium, ventricle and conus arterio- sus; endothelin-1 (ET-1) was demonstrated in subendo- cardial myocytes of the atrium and the conus. No ET-1 immunoreactivity was observed in the ventricle wall. At the light-microscopical level, ET-1 appeared to occur in the endocardium, but at the electron-microscopical level no immunogold labelling was seen on the granules of the endocardial cells. It is suggested that ET-1 is pro- duced and stored in the subendocardial cells and re- leased into the subendocardial space to reach the ANP- producing myocytes and the endothelial cells.
Immunoreactive localization of vasoactive hormones (Atrial natriuretic peptide and endothelin) in the heart of Protopterus annectens, an African lungfish
MASINI, MARIA ANGELA
Primo
Membro del Collaboration Group
;
1996-01-01
Abstract
The present study demonstrated, by immuno- histochemistry and Western blotting, the presence of im- munoreacting atrial natriuretic peptide (ANP) and endo- thelin in the heart of Protopterus annectens by both light and electron microscopy. The distribution of ANP gran- ules was investigated. ANP granules were localised in myocytes from the atrium, ventricle and conus arterio- sus; endothelin-1 (ET-1) was demonstrated in subendo- cardial myocytes of the atrium and the conus. No ET-1 immunoreactivity was observed in the ventricle wall. At the light-microscopical level, ET-1 appeared to occur in the endocardium, but at the electron-microscopical level no immunogold labelling was seen on the granules of the endocardial cells. It is suggested that ET-1 is pro- duced and stored in the subendocardial cells and re- leased into the subendocardial space to reach the ANP- producing myocytes and the endothelial cells.File | Dimensione | Formato | |
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