Genomic DNA extraction is a primary component for genomic research and diagnostic routine analyses. Recently, the importance of this process has been highlighted by necessity to standardize the diagnostic procedure. On this regard, the minimal residual disease (MRD) network of the Fondazione Italiana Linfomi (FIL MRD Network) has performed a comparative study of four different commercially available kits for DNA extraction applying them on a panel of cellular pellets with the aim to define possible technical recommendations in order to harmonize and standardize the diagnostic procedures in the clinical setting. Overall, all four kits most often allowed the recovery of a significant quantity of high quality DNA (in most conditions) although, specific indications could be addressed for cellular pellets of different size.
Comparison of different DNA extraction methods from peripheral blood cells: advices from the Fondazione Italiana Linfomi - MRD Network.
Ladetto M;GAIDANO, Gianluca;
2016-01-01
Abstract
Genomic DNA extraction is a primary component for genomic research and diagnostic routine analyses. Recently, the importance of this process has been highlighted by necessity to standardize the diagnostic procedure. On this regard, the minimal residual disease (MRD) network of the Fondazione Italiana Linfomi (FIL MRD Network) has performed a comparative study of four different commercially available kits for DNA extraction applying them on a panel of cellular pellets with the aim to define possible technical recommendations in order to harmonize and standardize the diagnostic procedures in the clinical setting. Overall, all four kits most often allowed the recovery of a significant quantity of high quality DNA (in most conditions) although, specific indications could be addressed for cellular pellets of different size.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
