In-vitro cell-based assays are widely used to characterize a molecule's bioactivity or quantify the potency of clinical and marketed drugs, as requested by regulatory authorities. Fetal Bovine Serum (FBS) is the gold standard of cell supplements but at the same time, it is one of the most critical reagents for cell culture. Firstly, FBS collection causes suffering to animals. Secondly, FBS is one of the higher sources of cell culture contamination. Finally, FBS composition is not completely characterized, and this could lead to batch-to-batch variability. Therefore, the idea is to adapt the HEK-293T cells to grow in a homemade Serum-Free Medium (SFM) where each media component is known, chemically characterized, and replaceable by the user.
Serum Free Medium
Vigna V.;
2024-01-01
Abstract
In-vitro cell-based assays are widely used to characterize a molecule's bioactivity or quantify the potency of clinical and marketed drugs, as requested by regulatory authorities. Fetal Bovine Serum (FBS) is the gold standard of cell supplements but at the same time, it is one of the most critical reagents for cell culture. Firstly, FBS collection causes suffering to animals. Secondly, FBS is one of the higher sources of cell culture contamination. Finally, FBS composition is not completely characterized, and this could lead to batch-to-batch variability. Therefore, the idea is to adapt the HEK-293T cells to grow in a homemade Serum-Free Medium (SFM) where each media component is known, chemically characterized, and replaceable by the user.| File | Dimensione | Formato | |
|---|---|---|---|
|
EP4342977A1_Original_document_20240416110123.zip
file sotto embargo fino al 31/10/2025
Tipologia:
Altro materiale allegato
Licenza:
DRM non definito
Dimensione
2.64 MB
Formato
Zip File
|
2.64 MB | Zip File | Visualizza/Apri Richiedi una copia |
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
