Cu-doped bioactive glass with enhanced in vitro bioactivity and antibacterial properties

This work aimed to optimize, produce and characterize Cu-doped bioactive glasses which are antibacterial without the addition of antibiotics obtained via ion exchange in an aqueous solution. According to morphological, compositional and structural analyses, 0.001 M was selected as the most optimal concentration of the ion exchange solution. The doped glass was then compared to the undoped one to investigate the effect of Cu-doping on the glass surface composition and bioactivity. Cu-doping was found to enhance the bioactivity kinetics and the following hydroxyapatite formation, evidenced by X-ray diffraction, energy dispersive X-ray spectroscopy, and zeta potential measurements. Besides that, the zeta potential titration measurements confirmed that the Cu-doping did not alter the surface chemical stability of the glass both in the inflammatory and physiological pH range. Moreover, the leaching ability of Cu2+-ions, both in physiological and inflammatory-mimicking conditions, was measured, followed by an in-depth study of the antibacterial properties, using two protocols to distinguish between the antiadhesive, antibacterial, and antibiofilm effects. For both protocols, a reduction of metabolic activity and Colony-Forming Unit after 24 h against Staphylococcus aureus Multi-Drug resistance strain was evidenced. These results showed that Cu-doped glass could show potential as a bioactive and antibacterial surface for bone surgery applications.