In recent years Italy experienced a strong migration flow from Albania: a STR reference database, to be used in forensic casework, was created for the Albanian population. Blood/saliva samples were obtained from 100 unrelated Albanians residing in Piedmont (North West Italy). Genomic DNA was isolated from blood by the Chelex method (1) and from saliva by standard organic phenol/chlorophorm extraction. PCR amplification was performed according to the manufacturer's instructions, using the GenePrint™ PowerPlex™ System (Promega Corporation, Madison, WI). The amplified products were detected with the 310 Genetic Analyzer (Perkin-Elmer, Foster City, CA). Hardy-Weinberg equilibrium was evaluated by exact test using the software GENEPOP Version 3.2a (2). The complete data are available to any interested researcher at http://medicina.medfarm.unito.it/dipart/dafml/torre/databasealbanians.htm
Allele frequencies for the PowerPlex 16 STR loci in an Albanian population sample from northern Italy
GINO Sarah;
2001-01-01
Abstract
In recent years Italy experienced a strong migration flow from Albania: a STR reference database, to be used in forensic casework, was created for the Albanian population. Blood/saliva samples were obtained from 100 unrelated Albanians residing in Piedmont (North West Italy). Genomic DNA was isolated from blood by the Chelex method (1) and from saliva by standard organic phenol/chlorophorm extraction. PCR amplification was performed according to the manufacturer's instructions, using the GenePrint™ PowerPlex™ System (Promega Corporation, Madison, WI). The amplified products were detected with the 310 Genetic Analyzer (Perkin-Elmer, Foster City, CA). Hardy-Weinberg equilibrium was evaluated by exact test using the software GENEPOP Version 3.2a (2). The complete data are available to any interested researcher at http://medicina.medfarm.unito.it/dipart/dafml/torre/databasealbanians.htmI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
