The Animal Type Pig PCR Amplification kit (Biotype AG, Dresden, Germany), a multiplex PCR system that allows the simultaneous amplification of 11 tetrameric short tandem repeats (STRs) and an Amelogenin-like locus for individual identification and sexing of pig (sus scrofa), was employed in the investigation of an alleged case of veterinary malpractice. A veterinarian responsible for collecting random blood samples as part of the program for the serological surveillance of swine vesicular disease (SVD) was accused of having drawn multiple samples from a single sow. STR typing of serum left overs from SVD testing strongly supported the conclusion that all the questioned samples included DNA from the same animal.
Forensic application of a multiplex PCR system for the typing of pig STRs
GINO Sarah;
2008-01-01
Abstract
The Animal Type Pig PCR Amplification kit (Biotype AG, Dresden, Germany), a multiplex PCR system that allows the simultaneous amplification of 11 tetrameric short tandem repeats (STRs) and an Amelogenin-like locus for individual identification and sexing of pig (sus scrofa), was employed in the investigation of an alleged case of veterinary malpractice. A veterinarian responsible for collecting random blood samples as part of the program for the serological surveillance of swine vesicular disease (SVD) was accused of having drawn multiple samples from a single sow. STR typing of serum left overs from SVD testing strongly supported the conclusion that all the questioned samples included DNA from the same animal.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
